OperaV1, Main, Exploration, bibRecord, 000008

Two-Photon Excitation STED Microscopy with Time-Gated Detection.

Identifieur interne : 000008 ( Main/Exploration ); précédent : 000007; suivant : 000009

Two-Photon Excitation STED Microscopy with Time-Gated Detection.

Auteurs : Iván Coto Hernández [Italie] ; Marco Castello [Italie] ; Luca Lanzan [Italie] ; Marta D'Amora [Italie] ; Paolo Bianchini [Italie] ; Alberto Diaspro [Italie] ; Giuseppe Vicidomini [Italie]

Source :

Scientific reports ; 2016.

RBID : pubmed:26757892

Abstract

We report on a novel two-photon excitation stimulated emission depletion (2PE-STED) microscope based on time-gated detection. The time-gated detection allows for the effective silencing of the fluorophores using moderate stimulated emission beam intensity. This opens the possibility of implementing an efficient 2PE-STED microscope with a stimulated emission beam running in a continuous-wave. The continuous-wave stimulated emission beam tempers the laser architecture's complexity and cost, but the time-gated detection degrades the signal-to-noise ratio (SNR) and signal-to-background ratio (SBR) of the image. We recover the SNR and the SBR through a multi-image deconvolution algorithm. Indeed, the algorithm simultaneously reassigns early-photons (normally discarded by the time-gated detection) to their original positions and removes the background induced by the stimulated emission beam. We exemplify the benefits of this implementation by imaging sub-cellular structures. Finally, we discuss of the extension of this algorithm to future all-pulsed 2PE-STED implementationd based on time-gated detection and a nanosecond laser source.

DOI: 10.1038/srep19419
PubMed: 26757892

Affiliations:

Italie

Le document en format XML

<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Two-Photon Excitation STED Microscopy with Time-Gated Detection.</title>
<author><name sortKey="Coto Hernandez, Ivan" sort="Coto Hernandez, Ivan" uniqKey="Coto Hernandez I" first="Iván" last="Coto Hernández">Iván Coto Hernández</name>
<affiliation wicri:level="1"><nlm:affiliation>Nanoscopy, Nanophysics, Istituto Italiano di Tecnologia, Via Morego 30, 16163 Genoa, Italy.</nlm:affiliation>
<country xml:lang="fr">Italie</country>
<wicri:regionArea>Nanoscopy, Nanophysics, Istituto Italiano di Tecnologia, Via Morego 30, 16163 Genoa</wicri:regionArea>
<wicri:noRegion>16163 Genoa</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Castello, Marco" sort="Castello, Marco" uniqKey="Castello M" first="Marco" last="Castello">Marco Castello</name>
<affiliation wicri:level="1"><nlm:affiliation>Nanoscopy, Nanophysics, Istituto Italiano di Tecnologia, Via Morego 30, 16163 Genoa, Italy.</nlm:affiliation>
<country xml:lang="fr">Italie</country>
<wicri:regionArea>Nanoscopy, Nanophysics, Istituto Italiano di Tecnologia, Via Morego 30, 16163 Genoa</wicri:regionArea>
<wicri:noRegion>16163 Genoa</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Lanzan, Luca" sort="Lanzan, Luca" uniqKey="Lanzan L" first="Luca" last="Lanzan">Luca Lanzan</name>
<affiliation wicri:level="1"><nlm:affiliation>Nanoscopy, Nanophysics, Istituto Italiano di Tecnologia, Via Morego 30, 16163 Genoa, Italy.</nlm:affiliation>
<country xml:lang="fr">Italie</country>
<wicri:regionArea>Nanoscopy, Nanophysics, Istituto Italiano di Tecnologia, Via Morego 30, 16163 Genoa</wicri:regionArea>
<wicri:noRegion>16163 Genoa</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="D Amora, Marta" sort="D Amora, Marta" uniqKey="D Amora M" first="Marta" last="D'Amora">Marta D'Amora</name>
<affiliation wicri:level="1"><nlm:affiliation>Nanoscopy, Nanophysics, Istituto Italiano di Tecnologia, Via Morego 30, 16163 Genoa, Italy.</nlm:affiliation>
<country xml:lang="fr">Italie</country>
<wicri:regionArea>Nanoscopy, Nanophysics, Istituto Italiano di Tecnologia, Via Morego 30, 16163 Genoa</wicri:regionArea>
<wicri:noRegion>16163 Genoa</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Bianchini, Paolo" sort="Bianchini, Paolo" uniqKey="Bianchini P" first="Paolo" last="Bianchini">Paolo Bianchini</name>
<affiliation wicri:level="1"><nlm:affiliation>Nanoscopy, Nanophysics, Istituto Italiano di Tecnologia, Via Morego 30, 16163 Genoa, Italy.</nlm:affiliation>
<country xml:lang="fr">Italie</country>
<wicri:regionArea>Nanoscopy, Nanophysics, Istituto Italiano di Tecnologia, Via Morego 30, 16163 Genoa</wicri:regionArea>
<wicri:noRegion>16163 Genoa</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Diaspro, Alberto" sort="Diaspro, Alberto" uniqKey="Diaspro A" first="Alberto" last="Diaspro">Alberto Diaspro</name>
<affiliation wicri:level="1"><nlm:affiliation>Nanoscopy, Nanophysics, Istituto Italiano di Tecnologia, Via Morego 30, 16163 Genoa, Italy.</nlm:affiliation>
<country xml:lang="fr">Italie</country>
<wicri:regionArea>Nanoscopy, Nanophysics, Istituto Italiano di Tecnologia, Via Morego 30, 16163 Genoa</wicri:regionArea>
<wicri:noRegion>16163 Genoa</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Vicidomini, Giuseppe" sort="Vicidomini, Giuseppe" uniqKey="Vicidomini G" first="Giuseppe" last="Vicidomini">Giuseppe Vicidomini</name>
<affiliation wicri:level="1"><nlm:affiliation>Nanoscopy, Nanophysics, Istituto Italiano di Tecnologia, Via Morego 30, 16163 Genoa, Italy.</nlm:affiliation>
<country xml:lang="fr">Italie</country>
<wicri:regionArea>Nanoscopy, Nanophysics, Istituto Italiano di Tecnologia, Via Morego 30, 16163 Genoa</wicri:regionArea>
<wicri:noRegion>16163 Genoa</wicri:noRegion>
</affiliation>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">PubMed</idno>
<date when="2016">2016</date>
<idno type="doi">10.1038/srep19419</idno>
<idno type="RBID">pubmed:26757892</idno>
<idno type="pmid">26757892</idno>
<idno type="wicri:Area/PubMed/Corpus">000021</idno>
<idno type="wicri:Area/PubMed/Curation">000021</idno>
<idno type="wicri:Area/PubMed/Checkpoint">000004</idno>
<idno type="wicri:Area/Ncbi/Merge">000E71</idno>
<idno type="wicri:Area/Ncbi/Curation">000E71</idno>
<idno type="wicri:Area/Ncbi/Checkpoint">000E71</idno>
<idno type="wicri:Area/Main/Merge">000008</idno>
<idno type="wicri:Area/Main/Curation">000008</idno>
<idno type="wicri:Area/Main/Exploration">000008</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title xml:lang="en">Two-Photon Excitation STED Microscopy with Time-Gated Detection.</title>
<author><name sortKey="Coto Hernandez, Ivan" sort="Coto Hernandez, Ivan" uniqKey="Coto Hernandez I" first="Iván" last="Coto Hernández">Iván Coto Hernández</name>
<affiliation wicri:level="1"><nlm:affiliation>Nanoscopy, Nanophysics, Istituto Italiano di Tecnologia, Via Morego 30, 16163 Genoa, Italy.</nlm:affiliation>
<country xml:lang="fr">Italie</country>
<wicri:regionArea>Nanoscopy, Nanophysics, Istituto Italiano di Tecnologia, Via Morego 30, 16163 Genoa</wicri:regionArea>
<wicri:noRegion>16163 Genoa</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Castello, Marco" sort="Castello, Marco" uniqKey="Castello M" first="Marco" last="Castello">Marco Castello</name>
<affiliation wicri:level="1"><nlm:affiliation>Nanoscopy, Nanophysics, Istituto Italiano di Tecnologia, Via Morego 30, 16163 Genoa, Italy.</nlm:affiliation>
<country xml:lang="fr">Italie</country>
<wicri:regionArea>Nanoscopy, Nanophysics, Istituto Italiano di Tecnologia, Via Morego 30, 16163 Genoa</wicri:regionArea>
<wicri:noRegion>16163 Genoa</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Lanzan, Luca" sort="Lanzan, Luca" uniqKey="Lanzan L" first="Luca" last="Lanzan">Luca Lanzan</name>
<affiliation wicri:level="1"><nlm:affiliation>Nanoscopy, Nanophysics, Istituto Italiano di Tecnologia, Via Morego 30, 16163 Genoa, Italy.</nlm:affiliation>
<country xml:lang="fr">Italie</country>
<wicri:regionArea>Nanoscopy, Nanophysics, Istituto Italiano di Tecnologia, Via Morego 30, 16163 Genoa</wicri:regionArea>
<wicri:noRegion>16163 Genoa</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="D Amora, Marta" sort="D Amora, Marta" uniqKey="D Amora M" first="Marta" last="D'Amora">Marta D'Amora</name>
<affiliation wicri:level="1"><nlm:affiliation>Nanoscopy, Nanophysics, Istituto Italiano di Tecnologia, Via Morego 30, 16163 Genoa, Italy.</nlm:affiliation>
<country xml:lang="fr">Italie</country>
<wicri:regionArea>Nanoscopy, Nanophysics, Istituto Italiano di Tecnologia, Via Morego 30, 16163 Genoa</wicri:regionArea>
<wicri:noRegion>16163 Genoa</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Bianchini, Paolo" sort="Bianchini, Paolo" uniqKey="Bianchini P" first="Paolo" last="Bianchini">Paolo Bianchini</name>
<affiliation wicri:level="1"><nlm:affiliation>Nanoscopy, Nanophysics, Istituto Italiano di Tecnologia, Via Morego 30, 16163 Genoa, Italy.</nlm:affiliation>
<country xml:lang="fr">Italie</country>
<wicri:regionArea>Nanoscopy, Nanophysics, Istituto Italiano di Tecnologia, Via Morego 30, 16163 Genoa</wicri:regionArea>
<wicri:noRegion>16163 Genoa</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Diaspro, Alberto" sort="Diaspro, Alberto" uniqKey="Diaspro A" first="Alberto" last="Diaspro">Alberto Diaspro</name>
<affiliation wicri:level="1"><nlm:affiliation>Nanoscopy, Nanophysics, Istituto Italiano di Tecnologia, Via Morego 30, 16163 Genoa, Italy.</nlm:affiliation>
<country xml:lang="fr">Italie</country>
<wicri:regionArea>Nanoscopy, Nanophysics, Istituto Italiano di Tecnologia, Via Morego 30, 16163 Genoa</wicri:regionArea>
<wicri:noRegion>16163 Genoa</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Vicidomini, Giuseppe" sort="Vicidomini, Giuseppe" uniqKey="Vicidomini G" first="Giuseppe" last="Vicidomini">Giuseppe Vicidomini</name>
<affiliation wicri:level="1"><nlm:affiliation>Nanoscopy, Nanophysics, Istituto Italiano di Tecnologia, Via Morego 30, 16163 Genoa, Italy.</nlm:affiliation>
<country xml:lang="fr">Italie</country>
<wicri:regionArea>Nanoscopy, Nanophysics, Istituto Italiano di Tecnologia, Via Morego 30, 16163 Genoa</wicri:regionArea>
<wicri:noRegion>16163 Genoa</wicri:noRegion>
</affiliation>
</author>
</analytic>
<series><title level="j">Scientific reports</title>
<idno type="e-ISSN">2045-2322</idno>
<imprint><date when="2016" type="published">2016</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
</fileDesc>
<profileDesc><textClass></textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en">We report on a novel two-photon excitation stimulated emission depletion (2PE-STED) microscope based on time-gated detection. The time-gated detection allows for the effective silencing of the fluorophores using moderate stimulated emission beam intensity. This opens the possibility of implementing an efficient 2PE-STED microscope with a stimulated emission beam running in a continuous-wave. The continuous-wave stimulated emission beam tempers the laser architecture's complexity and cost, but the time-gated detection degrades the signal-to-noise ratio (SNR) and signal-to-background ratio (SBR) of the image. We recover the SNR and the SBR through a multi-image deconvolution algorithm. Indeed, the algorithm simultaneously reassigns early-photons (normally discarded by the time-gated detection) to their original positions and removes the background induced by the stimulated emission beam. We exemplify the benefits of this implementation by imaging sub-cellular structures. Finally, we discuss of the extension of this algorithm to future all-pulsed 2PE-STED implementationd based on time-gated detection and a nanosecond laser source.</div>
</front>
</TEI>
<affiliations><list><country><li>Italie</li>
</country>
</list>
<tree><country name="Italie"><noRegion><name sortKey="Coto Hernandez, Ivan" sort="Coto Hernandez, Ivan" uniqKey="Coto Hernandez I" first="Iván" last="Coto Hernández">Iván Coto Hernández</name>
</noRegion>
<name sortKey="Bianchini, Paolo" sort="Bianchini, Paolo" uniqKey="Bianchini P" first="Paolo" last="Bianchini">Paolo Bianchini</name>
<name sortKey="Castello, Marco" sort="Castello, Marco" uniqKey="Castello M" first="Marco" last="Castello">Marco Castello</name>
<name sortKey="D Amora, Marta" sort="D Amora, Marta" uniqKey="D Amora M" first="Marta" last="D'Amora">Marta D'Amora</name>
<name sortKey="Diaspro, Alberto" sort="Diaspro, Alberto" uniqKey="Diaspro A" first="Alberto" last="Diaspro">Alberto Diaspro</name>
<name sortKey="Lanzan, Luca" sort="Lanzan, Luca" uniqKey="Lanzan L" first="Luca" last="Lanzan">Luca Lanzan</name>
<name sortKey="Vicidomini, Giuseppe" sort="Vicidomini, Giuseppe" uniqKey="Vicidomini G" first="Giuseppe" last="Vicidomini">Giuseppe Vicidomini</name>
</country>
</tree>
</affiliations>
</record>

Pour manipuler ce document sous Unix (Dilib)

EXPLOR_STEP=$WICRI_ROOT/Wicri/Musique/explor/OperaV1/Data/Main/Exploration

HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000008 | SxmlIndent | more

HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 000008 | SxmlIndent | more

Pour mettre un lien sur cette page dans le réseau Wicri

{{Explor lien
   |wiki=    Wicri/Musique
   |area=    OperaV1
   |flux=    Main
   |étape=   Exploration
   |type=    RBID
   |clé=     pubmed:26757892
   |texte=   Two-Photon Excitation STED Microscopy with Time-Gated Detection.
}}

Pour générer des pages wiki

HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i   -Sk "pubmed:26757892" \
       | HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd   \
       | NlmPubMed2Wicri -a OperaV1

This area was generated with Dilib version V0.6.21.
Data generation: Thu Apr 14 14:59:05 2016. Site generation: Thu Jan 4 23:09:23 2024

Serveur d'exploration sur l'opéra

Two-Photon Excitation STED Microscopy with Time-Gated Detection.

Two-Photon Excitation STED Microscopy with Time-Gated Detection.

Source :

Abstract

Links toward previous steps (curation, corpus...)

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri

Pour générer des pages wiki

	Serveur d'exploration sur l'opéra
	Attention, ce site est en cours de développement ! Attention, site généré par des moyens informatiques à partir de corpus bruts. Les informations ne sont donc pas validées.